FITC-BIOTIN 荧光素标记生物素的参考文献

FITC-BIOTIN 是由异硫氰酸荧光素（FITC）和生物素通过共价键连接而成的荧光探针。该分子保留了生物素对亲和素/链霉亲和素的高亲和力，同时具有 FITC 的典型荧光特性（激发波长约495 nm，发射约519 nm）。FITC-BIOTIN 常用于免疫荧光、细胞成像、亲和标记、ELISA 检测及微阵列技术中。其结构稳定，水溶性良好，既具靶向识别能力又具荧光示踪功能，是分子探针和纳米载体修饰的优选标记分子。

一、简介

名称：FITC-Biotin

中文名称：荧光素标记生物素

英文别名：Fluorescein-Biotin，Fluorescein Isothiocyanate-Biotin，Biotin-FITC

性状：黄色粉末

结构：

应用说明：

生物素可用于：少突胶质细胞培养。作为芽孢杆菌(Bacillus)生长的维生素补充剂。免疫组织学操作方案中的内源性生物素阻断。生物素是一种小分子，而且它与生物活性大分子共存时并不会影响到大分子的生物学功能。生物素-（链霉）亲和素系统是多种应用所必需的。生物素还可作为各种羧化酶的辅因子/辅酶，如丙酮酸羧化酶、乙酰辅酶A羧化酶 1 和 2、3-甲基巴豆酰辅酶A羧化酶 (MCC) 和丙酰辅酶A羧化酶 (PCC)。它可催化丙酮酸和CO2形成草酰乙酸。与亲和素或链霉亲和素偶联的生物素可帮助将靶分子（抗体、核苷酸、蛋白A等）与标记系统（酶、荧光、化学发光探针）相连接。该复合物可用于多种检测系统，如免疫检测、DNA杂交检测、免疫组化和流式细胞术。该方法还可用于多种目标分子的纯化和表征。生物素参与基因、细胞信号传导和染色质结构的表观遗传调控。荧光素标记的生物素(FITC-Biotin)载体酶切:将表达质粒用限制性内切酶(同引物的酶切位点)进行双酶切,酶切产物行琼脂糖电泳后,用胶回收Kit或冻融法回收载体大片段。

参考文献：

来源：

https://pubs.acs.org/doi/abs/10.1021/nn901474y

主要描述：

We describe molecular capturing properties of protein nanotubes with a controllable ligand binding affinity and size selectivity. These practical biocylinders were prepared using an alternating layer-by-layer (LbL) assembly of protein and oppositely charged poly(amino acid) into the nanoporous polycarbonate (PC) membrane (pore diameter, 400 nm), with subsequent dissolution of the template. The tube wall typically comprises six layers of poly-l-arginine (PLA) and human serum albumin (HSA) [(PLA/HSA)3]. Use of high molecular weight PLA (Mw = ca. 70 000) yielded robust nanotubes, which are available as lyophilized powder. The (PLA/HSA)3 nanotubes swelled considerably in water, although the outer diameter was almost unaltered. Uranyl ion, 3,3′-diethylthiacarbocyanine iodide, and zinc(II) protoporphyrin IX (ZnPP) were bound to the HSA component in the cylinder wall. Similar nanotubes comprising recombinant HSA mutant [rHSA(His)], which has a strong binding affinity for ZnPP, captured this ligand more tightly. Furthermore, addition of excess myristic acid released ZnPP from the tubes through a ligand replacement reaction. The hybrid nanotubes bearing a single avidin layer as an internal surface captured FITC-biotin efficiently. Biotin-labeled nanoparticles are also incorporated into the tubes when their particle size is sufficiently small to enter the pores. Subsequent TEM observation revealed a line of loaded nanoparticles (100 nm) in the one-dimensional space interior.