产品分类
快速订购
31952518
  • 029-86354885
  • 13309186942
首页 > 资料专栏

(SPDP) CAS #: 68181-17-9 产品使用说明书(含氨基马来酰亚胺蛋白)

时间:2018-03-30 14:54:23       浏览:3316

(SPDP) CAS #: 68181-17-9 产品使用说明书(含氨基马来酰亚胺蛋白)


SPDPCAS #: 68181-17-9 产品可以偶连带氨基的蛋白和巯基或马来酰亚胺的蛋白,偶连带活性基团的蛋白示意图:

 

我们可以提供三种不同结构的SPDP异端功能蛋白交联剂:SPDP,LC-SPDP,Sulfo-LC-SPDP

其中SPDP,LC-SPDP是脂溶性蛋白交联剂,Sulfo-LC-SPDP是水溶性蛋白交联剂。

 

Procedure for Conjugating Two Proteins, Oneof Which Contains Sulfhydryls

 

To conjugate between primary amines on oneprotein and sulfhydryls (-SH) already present on a second protein, only thefirst protein must be modified with the SPDP reagent.2 Then, after desaltingthe SPDP-modified protein to remove reaction byproducts and excess nonreactedreagent, the sulfhydryl-containing protein is added to make the final conjugate(Figure 2, Reaction 1)

 

Additional Materials Required

 

Water-miscible organic solvent such asdimethylsulfoxide (DMSO) or

dimethylformamide (DMF); not necessary forSulfo-LC-SPDP

 

Phosphate buffered saline with EDTA(PBS-EDTA): 100mM sodium phosphate, 150mM NaCl, 1mM EDTA, 0.02% sodium azide,pH 7.5

 

Proteins to be conjugated: In theprocedure, IgG and β-galactosidase represent the amine-containing andsulfhydrylcontaining protein, respectively.

 

Procedure

Equilibrate the vial of SPDP Reagent toroom temperature before opening. 2.

 

Immediately before use, prepare a 20mMsolution of SPDP Reagent:

 

Add 25µL of the 20mM SPDP solution to 2-5mgIgG dissolved in 1.0mL of PBS-EDTA.

 

Incubate for 30 minutes at roomtemperature.

 

Equilibrate a desalting column withPBS-EDTA, and buffer exchange the SPDP-modified IgG to remove reactionbyproducts and excess nonreacted SPDP reagent

 

Add 1-3mg of β-galactosidase (~1-3 molesβ-gal per mole of IgG) to the IgG solution and incubate the reaction mixtureovernight at room temperature

 

The proteins (IgG and β-galactosidase inthis example) are now conjugated.

 

Additional Information

 

Please visit the web site for additionalinformation on this product including the following items:

• Tech Tip #1: Attach a protein onto glass,silica or quartz surface using a cleavable crosslinker

• Tech Tip #26: Prepare a reversible andmeasureable sulfhydryl-reactive affinity column

 

Protocol For Pyridine-2-Thione Assay toDetermine Level of SPDP-modification

 

1. Dilute 100µL of SPDP-modified anddesalted protein to 1mL with PBS.

2. Measure and record the absorbance at343nm of the protein sample compared to PBS-EDTA blank (test in triplicate)

3. Add 10µL of 15mg/mL DTT to the 1mLSPDP-modified protein sample, mix.

4. After exactly 15 minutes, measure andrecord the absorbance at 343nm of the reduced sample.

5. Calculate the change in absorbance: ∆A343= (Ave. A343 after DTT) (Ave. A343 before DTT)

6. Calculate molar ratio of SPDP to Proteinusing the following equation:

 

Where the value 8080 reflects theextinction coefficient for pyridine-2-thione at 343 nm: 8.08 x 103 M-1 cm-1

 

 

产品名称:SPDP

英文名称:N-Succinimidyl3-(2-Pyridyldithio)propionate

中文名称:3-(2-吡啶基二硫基)丙酸N-羟基琥珀酰亚胺酯

CAS#: 68181-17-9

简介:SPDP crosslinker is a cleavable, waterinsoluble, amino and thiol (sulfhydryl) reactive heterobi-functional proteincrosslinker. The SPDP crosslinking reagent's reactive moites consist of anN-hydroxysuccinimide (NHS) ester that reacts with primary amines. On the otherend of SPDP, the pyridinyldisulfide reacts with sulhydryls to yield areversible disulfide bond.

SPDP交联剂是可分解的,不溶于水,氨和硫醇(巯基)反应heterobi功能蛋白交联剂。SPDP交联试剂的反应的moitesN-羟基琥珀酰亚胺(NHS)酯与伯胺反应。在另一端的pyridinyldisulfideSPDP,反应sulhydryls产生可逆的二硫键。

Application

3-(2-Pyridyldithio)propionic acidN-hydroxysuccinimide ester (SPDP) is useful for the preparation of enzymeimmunoconjugates and hapten carrier molecule conjugates.

It has been used for antibody conjugation andas a protein cross-linker.

 

General description

3-(2-Pyridyldithio)propionic acidN-hydroxysuccinimide ester (SPDP) is a heterobifunctional cross-linking reagentwith amine and sulfhydryl reactivity. It is typically coupled initially tomolecules containing primary amines by amide bonds buffered at pH 7.5(6.5-8.5). Second coupling specific for molecules containing free sulfhydryl bythiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternately, this reagentmay be used as a protected thiolating reagent following initial coupling andreduction of linker. It incorporates four atom linker.

 

外观:白色粉末

MolecularWeight: 312.4

分子式:C12H12N2O4S2

CAS#: 68181-17-9

溶解:DMSO or DMF

SpacerLength: 6.8 Å

Alternative Name:3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester

纯度:98%+

保存:-20

厂家:西安瑞禧生物科技有限公司

 

产品名称:Sulfo-LC-SPDP

英文名称:(sulfosuccinimidyl6-[3-(2-pyridyldithio)propionamido]hexanoate)

简介:Sulfo-LC-SPDP crosslinking reagent isa cleavable, water soluble, amino and thiol (sulfhydryl) reactiveheterobifunctional protein crosslinker.

Sulfo-LC-SPDP crosslinker's reactive moitesconsist of an N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts withprimary amines. The other end of the Sulfo-LC-SPDP crosslinking moleculepyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

LC SPDP交联试剂磺可裂解、水溶性氨基和巯基反应异型双功能交联剂(巯基)蛋白。

SPDP交联剂的反应moitesLC组成的N-羟基(磺基NHS)酯与伯胺反应。的LCSPDP交联分子pyridinyldisulfide磺化反应sulhydryls产生可逆的二硫键的另一端。

Caution

This product is sensitive to moisture. Thevial is packaged in a resealable bag with a desiccant to reduce exposure tomoisture. After cold storage, equilibrate the vial to room temperature beforeopening to reduce condensation inside the vial. Make fresh solutions. Storageof stock solutions is not recommended. After use, return the vial to theresealable bag. Close the bag and store the product at the recommendedtemperature.

General description

The SPDP reagents are a unique group ofamine- and sulfhydryl-reactive heterobifunctional crosslinkers. Whether theyare used to form amine-to-amine or amine-to-sulfhydryl crosslinks amongmolecules, the SPDP reagents produce disulfide-containing linkages that can becleaved later with reducing agents such as dithiothreitol (DTT).

 

外观:白色粉末

MolecularWeight: 527.6

分子式:C14H16N3NaO8S3

溶解:水中

SpacerLength: 15.7 Å

Alternative Name:Sulfosuccinimidyl-6-(3’-[2-pyridyldithio]-propionamido) hexanoate

纯度:98%+

保存:-20

厂家:西安瑞禧生物科技有限公司

 

产品列表:

Sulfo-NHSN-羟基琥珀酰亚胺磺酸钠盐,CAS#: 106627-54-7

Biotin-ONP(+)-生物素-4-硝基苯酯,CAS#: 33755-53-2

Biotin-PFP,五氟苯酚生物素酯/生物素五氟苯酯,CAS#:120550-35-8

6-(马来酰亚胺基)己酸琥珀酰亚胺酯,CAS#:55750-63-5

6-Maleimidohexanoic acidCAS#55750-53-3

N-Maleoyl-β-alanineMPA),CAS#7423-55-4

4-Maleimidobutyric acidMBA),CAS#57078-98-5

11-马来酰亚胺十一烷酸 N-琥珀酰亚胺酯,CAS#87981-04-2

2-马来酰亚胺基乙酸,CAS#25021-08-3

11-Maleimidoundecanoic acidKMUACAS#57079-01-3

Sulfo-EMCS, ε-马来酰亚胺己酸磺基琥珀酰亚胺酯  CAS#215312-86-0

AMAS, CAS#55750-61-3

1,2-Bis(maleimido)ethane, CAS#5132-30-9

Boc-6-Ahx-OSu, CAS#51513-80-5

N-Boc-2,2-(ethylenedioxy)diethylamine ,CAS#153086-78-3

N,N,N,N-Tetramethyl-p-phenylenediamine,CAS#100-22-1

N-Succinimidyl 6-Biotinamidohexanoate, CAS#72040-63-2

N-(+)-Biotinyl-6-aminohexanoic acid  CAS#72040-64-3

CAS#127062-22-0

DTSSP

BS3  CAS#: 82436-77-9

BS2G

BSG             CAS#: 79642-50-5

DSP        CAS#: 57757-57-0

DSS        CAS#: 68528-80-3

DTSSP    CAS#: 81069-02-5

EGS       CAS#: 70539-42-3

Sulfo-EGS    

DSSeb    CAS# 23024-29-5

BMPS     CAS#: 55750-62-4

EMCS     CAS#: 55750-63-5

GMBS    CAS#: 80307-12-6

LC-SPDP      

MBS       CAS#: 58626-38-3

PDPH           

SBA       CAS#: 42014-51-7

SIA        CAS#: 39028-27-8

SMCC     CAS#: 64987-85-5

SMPB     CAS#: 79886-55-8

SMPH    

SPDP      CAS#: 68181-17-9

Sulfo-LC-SPDP    

Sulfo-MBS

Sulfo-SANPAH            CAS #: 102568-43-4

Sulfo-SMCC                 CAS#: 92921-24-9

Biotin Hydrazide           CAS #: 66640-86-6

NHS-Biotin                  CAS#: 35013-72-0

NHS-SS-Biotin             CAS #: 142439-92-7

Sulfo-NHS-Biotin

HPG                            CAS#: 24645-80-5

SATA                           CAS#: 76931-93-6

Sulfo-NHS Acetate        CAS #: 152305-87-8

Biotin-maleimide         

Biotin ethylenediamine hydrobromide  CAS# 216299-38-6

Biotinamidohexanoic acid hydrazide     CAS#: 109276-34-8

CAS#: 89889-52-1

TSAT

BMPH           CAS#:359436-61-6

EMCH           CAS#:151038-94-7

KMUH           CAS#:1593661-78-9

BMOE           CAS:5132-30-9

BMB              CAS#:28537-70-4

BMH (bismaleimidohexane)         CAS#:4856-87-5

TMEA

DTME           CAS#:71865-37-7

SATP             CAS#:84271-78-3

EMCA           CAS:55750-53-3

AEDP           

 

以上资料源于西安瑞禧生物科技有限公司

如有其他信息或产品信息咨询请致电我们

西安瑞禧生物科技有限公司

Xi'an ruixi Biological Technology Co;Ltd

 

 


序号 新闻标题 浏览次数 作者 发布时间
1 抗氧化小分子70831-56-0,菊苣酸Cichoric Acid,6537-80-0的制备过程 409 瑞禧生物 2023-03-30
2 活性氧ROS小分子Dapsone,cas:80-08-0,氨苯砜的制备过程-瑞禧科研 350 瑞禧生物 2023-03-30
3 HBPS-N3,Azide-PEG-HBPS,叠氮化超支化聚苯乙烯高分子聚合物的制备过程 403 瑞禧生物 2023-03-17
4 l-PS-PhN3,Azide叠氮Azido偶联线性聚苯乙烯双链的制备过程 370 瑞禧生物 2023-03-17
5 N3-PS-N3,Azido-PS-Azido/Azide,双叠氮官能团修饰聚苯乙烯的制备方法 373 瑞禧生物 2023-03-17
6 PS-N3,Azido-PS,叠氮Azide修饰聚苯乙烯/高分子聚合物的制备过程 419 瑞禧生物 2023-03-17
7 Azido-PEG2-t-Butylester/1271728-79-0,叠氮N3/ZAD修饰叔丁酯化合物的制备方法 343 瑞禧生物 2023-03-14
合作品牌

库存查询