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Home > Products > Protein Crosslinkers> Other Crosslinkers> IPTG, CAS#:367-93-1
IPTG, CAS#:367-93-1
Catalog # Pkg Size Price Quantity Shopping Cart
R-S-9003 g ¥ 800
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结构式
外观 White powder
分子量 238.31
溶解度 有机溶剂或水
CAS号 367-93-1
质量控制 95%
储存条件 -20℃
保存时间 1 Year
其他信息 异丙基1-硫代-β-D-半乳糖吡喃糖苷

异丙基1-硫代-β-D-半乳糖吡喃糖苷(IPTG)是β–半乳糖苷酶的活性诱导物质,常用于蓝白斑筛选及IPTG诱导的细菌内的蛋白表达等。当pUC系列的载体DNA(或其他带有lacZ 基因载体DNA)以lacZ 缺失细胞为宿主进行转化时、或用M13噬菌体的载体DNA进行转染时,如果在平板培养基中加入X–Gal和IPTG,由于β–半乳糖苷酶的α–互补性,可以根据是否呈现白色菌落(或噬菌斑)而方便地挑选出基因重组体。此外,它还可以作为具有lac或tac等启动子的表达载体的表达诱导物使用。

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