MB™ 488 DBCO is a bright, green fluorescent probe with excitation ideally suited for the 488 nm laser line. MB™ 488 is bright, exceedingly photostable and pH-insensitive fluorescent dye that can be used in place of Fluorescein, Alexa Fluor 488®, DyLight 488® and other dyes excitable at a wavelength of 488 nm.

MB™ 488 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

MB™ 488 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.